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What did the authors conclude

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This further suggests that chemotherapy resistance may be related to the TME, and that the TME may even be the main cause of chemotherapy resistance [24]

Subsequently, the HUVECs are stained with a cell-permeable dye calcein AM (Corning, catalog number 354216) for 30 min, and then observed for capillary-like tubule formation using an inverted microscope