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By encapsulating amino acid-targeting agents, nanocarriers can enhance drug solubility, prolong plasma half-life, and facilitate selective drug delivery to tumor sites via passive or active targeting mechanisms, thereby maximizing therapeutic efficacy, while minimizing adverse effects on normal tissues [262, 263]

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Simultaneously, it increased the activities of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and ascorbate peroxidase (APX)

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For intracellular staining of hexokinase-1, cells were stained for extracellular markers, fixed in 4% FA at RT, and stained with Anti-Hexokinase 1 (Abcam ab184818) diluted 1:100 in PBS 0.1% Tween-20 at RT