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Next, we directly assessed whether the PVN Glu neurons contribute to these tonic currents in the DMV Ach neurons, specifically by ablating PVN Glu neurons based on infusion of a Cre-dependent genetically engineered caspase3 (AAV-DIO-taCaspase3) and AAV-DIO-EGFP viruses (or AAV-DIO-EGFP as a control) into the PVN, as well as FG injection into the small intestine of VgluT2-Cre mice (Supplementary Fig

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