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Transfection Min6 and NIT-1 cells were transfected with either pLJM1-MOTS-c, pLJM1-empty, pGenLenti- Cdkn2a (Genscript) or pGenLenti-empty (SH016)-vectors in 2.0 10 6 cells in six-well-plates using Neon transfection system (Thermo Fisher Scientific) as previous reported 6,7,8 or in RPMI containing polybrene (10 g ml 1

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However, staying attuned to systemic health trends is equally critical, especially with the rise of GLP-1 receptor agonists (like semaglutide and liraglutide) in managing diabetes and obesity