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Alveolar bone resorption and alveolar bone morphometric parameters were imaged at an isotropic voxelsize of 10 m, using a microCT Cone Beam 1172 SkyScan system (Skyscan, Kontich, Belgium) operating at a peak voltage of 100 kV and 100 A with a 0.5 mm aluminium filter

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Blots were blocked with phosphate-buffered saline with 0.1% Tween 20 and then bound with antibodies for Biotin, after which an enhanced chemiluminescence (ECL) reaction was performed for blot development

In medical trials, tesamorelin is typically prescribed at 2 mg once daily via subcutaneous injection

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